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KMID : 0903619920330040329
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Journal of the Korean Society for Horticultural Science
1992 Volume.33 No. 4 p.329 ~ p.336
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Mass Propagation of Betula pendula ´ Trost Dwarf ´ through Axillary Buds in Vitro
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심경구/Shim, Kyung Ku
하유미/이석구/Ha, Yoo Mi/Lee, Suk Koo
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Abstract
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To establish a rapid micropropagation method, axillary buds of Betula pendula ´Trost Dwarf´ were cultured on the woody plant medium (WPM), broad-leaved trees medium (BTM), Murashige & Skoog´s medium (MS), Gress-hoff & Doy´s medium (GD) supplemented with benzylaminopurine (BAP), Kinetin, naphthalene acetic acid (NAA) and indole butyric acid (IBA). The results are summarized as follows:
Etiolation was required for shoot elongation from axillary buds and resulted in shoot elongation and induction of multiple shoot at the basal part of explants. WPM and BTM containing 0.5 ㎎/ℓ of BAP were adequate for multiple shoots formation by using etiolated explant. In the combination of media and plant growth regulators, multiple shoot formation and shoot elongation of Betula pendula ´Trost Dwarf was stimulated by WPM supplemented with 0.5 ㎎/ℓ BAP and 0.01 ㎎/ℓ NAA or 0.01 ㎎/ℓ IBA as the average shoot number 9.8 and 10.28, while the number of shoots in WPM without BAP was 1.80. A 100% rooting was achieved on GD and 1/2 GD medium supplemented with 0.2 ㎎/ℓ IBA. The survival rates after transplanting into a mixture of peat moss and perlite (1:1 v/v) was 95% and then established plantlets were moved into the greenhouse and the survival rate was 95%, too. Conclusively, after 5 weeks of culture, we have obtained over 1000 plants of Betula pendula ´Trost Dwarf 3´ by the culture of 10 axillary buds.
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